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Gradient PCR Optima 96 | Four E’s Scientific

Gradient PCR are essential laboratory equipment for researchers who run polymerase chain reaction (PCR) for sequencing, cloning, genotyping, mutagenesis, and many other applications. It features precise PCR annealing temperature gradient to easily optimize PCR assays in a single run.

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FEATURES

● Six Independent temperature Blocks with imported TEC modules provide precise Temperature control for PCR optimization

● Gradient PCR thermal cycler with excellent thermal gradient temperature accuracy (±0.1°C ).

● Independent front and rear air inlet and outlet duct design, which allows instruments to be placed close to each other, saving laboratory space.

● Ultra large fin-type heat sink with intelligent fan speed control technology provides low-noise heat dissipation.

● Segmented heating lid design adapts to all types of PCR tubes, strips and well plates by automatically adjusting the lid height

● The Lid comes with Intelligent auto-heating off function to reduce non-specific amplification by turning off the heating when the temperature of sample blocks is below 30°C.

● Faster Heating and cooling Rates (Maximum heating rate >5°C/s, maximum cooling rate >4°C/s)

● Provides excellent temperature uniformity and the gradient layout reduces edge effect

● Comes with USB interface at the front for easy gradient PCR protocol import & export. Also, Device software can be upgrade through USB

● Gradient PCR machine comes with Multiple Accounts and Access rights (Administrator, User Accounts, Visitor Accounts)

● High resolution, 7-inch LCD touchscreen panel for easy operation and monitoring

● Touchdown (TD) PCR Mode and Long Range PCR Modes are available

● Compatible with universal PCR tubes, PCR 8 strips plates and 96-well PCR plates


TEMPERATURE CONTROL

Model

Optima96

Sample Capacity

96-well plates, 12x PCR 8 strips, 96x 0.2 ml well plates

PCR reaction volume

10-100 µl

Heating lid temperature

30-110°C

Max. block   heating Rate

5°C/s

Max. block   cooling Rate

4 °C/s

Temperature uniformity

<0.5°C (20s after reaching 95°C )

Sample   temperature range

4°C-100°C

Temperature accuracy

±0.1°C (35 °C - 99.9°C)

Technology  (heating/Cooling)

Semiconductor   Peltier technology, 6 independent  blocks

Display resolution

0.1°C


GRADIENT

Maximum temperature

difference   between blocks

25°C (max. 5°C   between two adjacent blocks)

Temperature  difference between

two adjacent blocks

0.1°C-5.0°C

Gradient   temperature accuracy

±0.1°C (35°C - 99.9°C)

Gradient temperature uniformity

<0.5°C (20s   after reaching 95°C)


PROGRAM FUNCTION

LCD display

7" LCD touch screen, real-time display of PCR process,

can be used   while wearing gloves

Max. number of steps

No limit

Time increment / decrement

1-120s, for   long Range PCR  experiment

Temperature increment / decrement

0.1°C-10.0°C,   for Touchdown PCR

Maximum number of cycles

Unlimited


OTHER FEATURES

Tm  value   calculation function

Yes

Automatic pause/power-off protection

Yes

Communication    interface

Network   interface (LAN), USB2.0 interface, Wifi, Bluetooth  2.0

Storage

External USB   Drive and Internal Memory

Program   storage capacity

Up to 15,000   programs in the  instrument,

Unlimited   storage with USB flash drive


POWER SUPPLY

Input power

100-240V,50-60Hz

Max. power

750 V A

Max. current

10A

Noise

<45dB


OTHER FUNCTIONS

Specific amplification

The blocks do not start heating until the heating lid temperature reaches the set temperature so as to prevent sample evaporation and improve reaction specificity.

Template

Pre-stored multiple standard experiment templates, simplify the tedious programming  steps

Certification

CE, RoHS

Dimensions (lxWxH)

364x243x244mm

Net weight

8.7kg


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